Characterization of the Yeast Actin Patch Protein App1p Phosphatidate Phosphatase
نویسندگان
چکیده
منابع مشابه
Characterization of the yeast actin patch protein App1p phosphatidate phosphatase.
Yeast App1p is a phosphatidate phosphatase (PAP) that associates with endocytic proteins at cortical actin patches. App1p, which catalyzes the conversion of phosphatidate (PA) to diacylglycerol, is unique among Mg(2+)-dependent PAP enzymes in that its reaction is not involved with de novo lipid synthesis. Instead, App1p PAP is thought to play a role in endocytosis because its substrate and prod...
متن کاملThe Saccharomyces cerevisiae actin patch protein App1p is a phosphatidate phosphatase enzyme.
BACKGROUND Phosphatidate phosphatase (PAP) plays diverse roles in lipid metabolism and cell signaling. RESULTS A novel yeast PAP is identified as the actin patch protein encoded by APP1. CONCLUSION APP1 and other known genes (PAH1, DPP1, LPP1) are responsible for all detectable PAP activity in yeast. SIGNIFICANCE Identification of App1p as a PAP enzyme will facilitate the understanding of...
متن کاملYeast Pah1p phosphatidate phosphatase is regulated by proteasome-mediated degradation.
Yeast PAH1-encoded phosphatidate phosphatase is the enzyme responsible for the production of the diacylglycerol used for the synthesis of triacylglycerol that accumulates in the stationary phase of growth. Paradoxically, the growth phase-mediated inductions of PAH1 and phosphatidate phosphatase activity do not correlate with the amount of Pah1p; enzyme abundance declined in a growth phase-depen...
متن کاملCharacterization of the human LPIN1-encoded phosphatidate phosphatase isoforms.
The human LPIN1 gene encodes the protein lipin 1, which possesses phosphatidate (PA) phosphatase (3-sn-phosphatidate phosphohydrolase; EC 3.1.3.4) activity (Han, G.-S., Wu, W.-I., and Carman, G. M. (2006) J. Biol. Chem. 281, 9210-9218). In this work, we characterized human lipin 1 alpha, beta, and gamma isoforms that were expressed in Escherichia coli and purified to near homogeneity. PA phosph...
متن کاملTORC1 Regulates Pah1 Phosphatidate Phosphatase Activity via the Nem1/Spo7 Protein Phosphatase Complex
The evolutionarily conserved target of rapamycin complex 1 (TORC1) controls growth-related processes such as protein, nucleotide, and lipid metabolism in response to growth hormones, energy/ATP levels, and amino acids. Its deregulation is associated with cancer, type 2 diabetes, and obesity. Among other substrates, mammalian TORC1 directly phosphorylates and inhibits the phosphatidate phosphata...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2013
ISSN: 0021-9258
DOI: 10.1074/jbc.m112.449629